ANALYSIS OF GENETIC VARION OF 4-LIKE SUBUNIT 4-LIKE NADH DEHYDROGENASE GENE SEQUENCES IN ADES aegypti POPSET: 1831566147 USING RFLP IN SILICO

Abstract

In silico studies have the potential to accelerate discovery while reducing the need for expensive laboratory work. RFLP analysis was performed to see the genetic variation of the 4-like subunit NADH dehydrogenase gene. The purpose of this study was to determine the genetic variation of the 4-like subunit 4-like subunit NADH dehydrogenase gene sequence in Aedes aegepti PopSet: 1831566147 by using in silico RFLP. The genetic variation of the Aedes aegypti population was analyzed by in silico RFLP-PCR, that is, online using the benchling web page. Restriction was performed using the Ase I enzyme. The results obtained were from 27 sample DNA sequences, 24 of which were restricted by the Ase I enzyme, forming 2 fragments with sizes 114 and 125 bp. The other 3 samples were not restricted, still measuring 339 bp. The results of this study indicate that there are 2 genetic variations of the Aedes aegypti population. The percentage of genetic variation is 7.4%. This number of percentages indicates the level of genetic variation of Aedes aegypti is very low.