Analisis Variasi Genetik Sekuen Gen Surface Glycoprotein (S) Pada SARS –CoV-2 Popset : 1843471817 Menggunakan RFLP Secara In-Sillico

https://doi.org/10.24036/prosemnasbio/vol1/8

Authors

  • Karina Hukma Alya Putri Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
  • Kartika Puspita Sari Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
  • Khairunnisa Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
  • Monicha Yhuyhen Safitri Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
  • Afif Putra Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
  • Katon Agusdi Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
  • Fahrul Rozi Oktavian Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
  • Afifatul Achyar Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang

Keywords:

Surface glycoprotein (S) gene, SARS-CoV-2, RFLP in silico

Abstract

The Surface glycoprotein (S) gene is a protein encoding gene at the GU280_gp02 locus tag found in the severe acute respiratory syndrome organism coronavirus 2 (SARS-CoV2) virus. Wuhan-Hu-1 isolate, nat-host:Homo sapiens. The SARS-CoV-2 virus is a positive enveloped single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19), the structural proteins of SARS-CoV-2 including protein S are important targets for vaccine development, antibody therapy and antigen-based diagnostic tests. This study aimed to analyze genetic variation in the surface glycoprotein (S) gene sequence in SARS-CoV-2 NCBI PopSet: 1843471817 using in-silico RFLP. The RNA sequences were cut using 2 types of restriction enzymes, namely BamH1 and BglII. The results of in-silico RFLP showed that there were genetic variations on the restriction site of the BamHI enzyme (Alleles A1 and A2 with allele frequencies of 0.125 and 0.875) and BgIII (Alleles B1 and B2 with allele frequencies of 0.875 and 0.125).

Published

2021-09-01

How to Cite

Putri, K. H. A. ., Kartika Puspita Sari, Khairunnisa, Monicha Yhuyhen Safitri, Afif Putra, Katon Agusdi, Fahrul Rozi Oktavian, & Afifatul Achyar. (2021). Analisis Variasi Genetik Sekuen Gen Surface Glycoprotein (S) Pada SARS –CoV-2 Popset : 1843471817 Menggunakan RFLP Secara In-Sillico. Prosiding Seminar Nasional Biologi, 1(1), 44–52. https://doi.org/10.24036/prosemnasbio/vol1/8

Issue

Vol. 1 No. 1 (2021): PROSIDING SEMINAR NASIONAL BIOLOGI 1

Section

Articles
Copyright & Licensing

Copyright (c) 2021 Karina Hukma Alya Putri, Kartika Puspita Sari, Khairunnisa, Monicha Yhuyhen Safitri, Afif Putra, Katon Agusdi, Fahrul Rozi Oktavian, Afifatul Achyar

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted non-commercial use, distribution and reproduction in any medium 

Most read articles by the same author(s)

1 2 3 4 > >>